Hif1a western blot
WebWestern blot analysis of HIF1A in 50 µg cobalt chloride induced COS-7 nuclear extracts. Samples were incubated in HIF1A monoclonal antibody (Product # MA1-16504. FIGURE 1 Effects of oxygen concentration on growth, cell-death, gene expression and metabolism in human colonoids (A) . WebHIF-1 alpha Antibody (H1alpha67) [NB100-105]: Western blot (WB) analysis of lysates from Caki-1 cells which were left un-treated or were treated with 100-300 uM of CoCl 2 for 4-6 hours. The antibody detected …
Hif1a western blot
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WebKnockdown of HIF1A was achieved by transfecting MCF7 with HIF1A specific siRNAs (Silencer® select Product # S6541, S6539). Western blot analysis was performed using Nuclear enriched extracts from the HIF1A knockdown cells (treated with CoCl2, 150 µM for 48hrs) (lane 4), non-targeting scrambled siRNA transfected cells (treated with CoCl2, … WebI've blotted for HIF1a many times. It does work best in human cells with the BD antibody. Also, it can help to do a nuclear lysate rather than whole cell. This enriches for HIF1a.
Web1 de set. de 2011 · DOI: 10.1016/j.ab.2011.04.037. Abstract. Hypoxia-inducible factor-1α (HIF-1α) is a widely studied protein with significant biomedical impact. Care is needed to … Web23 de jul. de 2024 · Santa Cruz Biotechnology monoclonal antibodies can be used in assays including flow cytometry, immunoprecipitation, Western blot analysis, and immunostaining. Anti-HIF1a Antibody (28b) is recommended for use in the following applications: WB (Western blotting), IP (Immunoprecipitation), IF (Immunofluorescence), IHC(P) …
For this step you need a tissue culture incubator capable of monitoring and regulating oxygen levels. Alternatively, you can use a hypoxic chamber on a general tissue culture incubator. 1. Set tissue culture incubator or chamber to an oxygen level of 2% or less through the regulated addition of nitrogen gas. Follow … Ver mais WebHIF-1 alpha can be a challenging target to work with in western blot. HIF-1 alpha is only stabilized at O 2 concentrations below 5%. Under normoxic conditions, HIF-1 alpha has …
Web20 de jun. de 2024 · A. Western blot was first labelled for HIF (Left), then washed and re-blotted for Tubulin (right). This unedited western shows MCF7 and MDA-MB-231 parental cells cultured in 21% and 1% oxygen for 48 hours before lysis. B. Western blot was cut at approximately 80kDa and the top was labelled for HIF1 whilst the bottom was labelled for …
WebWestern Blot. Detection of Human HIF‑1 alpha /HIF1A by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 150 µM CoCl 2 for 16 hours. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat HIF-1a/HIF1A Antigen-affinity Purified Polyclonal Antibody, followed … date of birth certificate srinagarWebPolyclonal Antibody for studying HIF1A. Cited in 194 publications. Validated for Western Blotting. Highly specific and rigorously validated in-house, … date of birth certificate tamilnaduWeb15 de jul. de 2003 · Methods and materials: HIF-1alpha expression following in vitro hypoxia was measured in U87 MG glioblastoma cells by Western blot and flow cytometry. Cell … bizad charity runWeb8 de jun. de 2024 · BACKGROUND Ovarian cancer has the highest mortality rate among all female genital tumors because of its insidious onset and drug resistance. Hypoxia-inducible factor 1alpha (HIF-1alpha), one of the best-studied oncogenes, plays an important part in tumor adaptation to microenvironmental hypoxia and … bizache refrigeratorsWebWestern Blot Shows Human HIF-1 alpha/HIF1A Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell … bizacumen research pvt ltdWebNational Center for Biotechnology Information biza duty free manchesterWeb25 de jun. de 2024 · Transcriptional analysis was used to find the possible targets in osteoarthritis. Western blot analysis, RT-qPCR and immunofluorescence staining were used to detect the changes in gene and protein levels in the vitro experiment. The expression of HIF-1α was increased in human and mouse OA cartilage. biz advance now inc