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Pcrxl-topo

SpletPCR Gel Purification and Cloning (Invitrogen XL TOPO PCR Cloning Kit) Abstract: The Chemicals, Equipments & Supplies box on the right contains a list of materials used in … SpletpCR-XL-TOPO Parental vector for TOPO® TA cloning of long PCR products. Vector Map (Click image to enlarge) Search our products with this vector backbone We offer a wide …

TOPO XL PCR Cloning Kit - Thermo Fisher Scientific

Spletcloned into pCRXL-TOPO or pCR2.1-TOPO cloning vector individually (Invitrogen, USA). The colonies were selected on LB medium supplemented with kanamycin (50 mg/ml) after transformation into E.coli DH5a cells. The recombinant clones containing the in-dividual pectolytic genes from XW19 or XW121 were sequenced using an automatic DNA … SpletpCRII-TOPO (linearized) Linearized vector with 3'-T overhangs and bound topoisomerase, for TOPO® TA cloning of PCR products. Similar to pCR®2.1-TOPO® but with an SP6 promoter. Sequence Author: Thermo Fisher (Invitrogen) Open in SnapGene Try SnapGene for Free Download Plasmid Download SnapGene Viewer kid hockey socks cup combo https://login-informatica.com

Separate-component-stabilization system for protein and

Splet05. dec. 2015 · Chimeric Rhinoviruses Obtained via Genetic Engineering or Artificially Induced Recombination Are Viable Only if the Polyprotein Coding Sequence Derives from the Same ... SpletIntroduction The pCR®T7 TOPO® TA Expression Kits provide a highly efficient, 5-minute, one-step cloning strategy ("TOPO® Cloning") for the direct insertion of Taq polymerase … Splet26. avg. 2015 · The amplified PCR product was purified by agarose gel extraction using a QIAquick gel extraction kit (Qiagen) per the manufacturer's protocol. All RNA products were generated using T7 RNA polymerase and a T7 MEGAshortscript kit (Ambion) according to the manufacturer's instructions. is medicare and medicaid federally funded

Strategy for efficient generation of numerous full-length cDNA …

Category:Invitrogen™ TOPO™ XL-2 Complete PCR Cloning Kit, with One …

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Pcrxl-topo

TOPO™ TA Cloning™ Kit, Dual Promoter, with pCR™II-TOPO ... - US

Splet01. okt. 2002 · The product of the inverse PCR was gel extracted, cloned with the TOPO TA Cloning kit with vector pCRXL-TOPO (Invitrogen), and fully sequenced. Primers NppkI-F and NppkI-R were subsequently designed to target the full-length Type I ppk (Table 2 ). SpletThe pCR™Blunt II-TOPO™ vector used in this kit comes with lethal ccdB gene for efficient selection of blunt-ended PCR recombinants products generated bythermostable …

Pcrxl-topo

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Spletcloned into the pCRXL-TOPO vector (Invitrogen), yielding the pCatccdB construct. The cat-ccdB fragment was next amplified by PCR from pCatccdB using the primers Rev and Fwd (Table Splet了解TOPO PCR克隆技术的工作原理 了解它如何简单地在 5 分钟内实现 95% 的克隆效率。 选择适合您的 TOPO 克隆解决方案 高保真平末端 TOPO 克隆 适用于将经校正热稳定DNA聚合酶扩增的平末端 PCR 产物直接插入质粒载体。 现在增加25%的反应次数。 适用于亚克隆 适用于测序 TOPO-TA 克隆 适用于将经 Taq 聚合酶扩增的 PCR 产物直接插入质粒载体进行 …

Splet中国微生物资源共享平台,微生物菌种保藏中心,泰斯拓生物提供菌株,atcc菌株,菌株,质粒载体,慢病毒，细菌，真菌,支原体,衣原体,dsmz,ccug,atcc,分子技术试验,相关的技术服务,咨询电话0574-87917803 Splet中国微生物资源共享平台,微生物菌种保藏中心,泰斯拓生物提供菌株,atcc菌株,菌株,质粒载体,慢病毒，细菌，真菌,支原体,衣原体,dsmz,ccug,atcc,分子技术试验,相关的技术服务,咨询电话0574-87917803

SpletTOPO™ XL-2 完整 PCR 克隆试剂盒为高效克隆超长 PCR 产物（高达 13 kb）提供所有必要元素。该试剂盒使用线性化和拓扑异构酶 1 活化的 pCR™-XL-2-TOPO™ 载体，其兼容平末端 PCR 片段的克隆。Platinum™ SuperFi™ Green PCR 预混液包含在试剂盒中，可扩增长 PCR … SpletDownload pCR-XL-2-TOPO.dna file Download Plasmid Open in SnapGene SnapGene SnapGene is the easiest way to plan, visualize and document your everyday molecular …

Splet质粒载体网zlzt.com专业提供pCR-XL-TOPO-SLC24A1(human) ,产品编号为zl-008487,为科研单位及院所提供的质粒载体展示及购买平台,保藏了大量质粒载体,致力于完美质粒众筹,质粒合成,质粒构建,质粒提取,质粒保藏!

Splet01. apr. 2009 · A common prototypic ETEC fimbria, colonization factor antigen I (CFA/I), consists of a tip-localized minor adhesive subunit CfaE and the stalk-forming major subunit CfaB, both of which are... kid hugs copSpletpCR-XL-TOPO® allows direct selection of recombinants via disruption of the lethal E. coli gene, ccdB (Bernard et al., 1994). The vector contains the B gene fused to the C-terminus … is medicare and medical assistance the sameSpletRoom temperature ligation (5 min) using the pCRXL-TOPO (Invitrogen-Life Technologies Inc., Carlsbad, CA) plasmid was performed and DH5a (Invitrogen-Life Technologies Inc.) were transformed by heat shock. Kanamycin resistant colonies were PCR screened using the EGFssF and NEWPLCR2 primers and the colonies as template using the above … kid hosting wedding invitationsSplet20. feb. 2024 · The kit uses the linearized and topoisomerase 1-activated pCR™-XL-2-TOPO™ vector, which is compatible with the cloning of blunt-end PCR fragments. Amplification of long PCR fragments is enabled by Platinum™SuperFi™ Green PCR Master Mix, which is included in the kit. Topoisomerase I activation of the vector allows PCR … is medicare an hdhpSpletTOPO® XL PCR Cloning Kit Five-minute cloning of long (3–10 kb) PCR products Catalog numbers K4700-10, K4700-20, K4750-10, K4750-20, K7030-20 Publication part number 25 … kid huntingand fishing beddingSpletpCR -XL-TOPO 3.5 kb. Comments for pCR®-XL-TOPO®. 3519 nucleotides Lac promoter/operator region: bases 95-216 M13 Reverse priming site: bases 205-221 Lac Za … kid how to put football gear onSpletThe TOPO XL-2 Complete PCR Cloning Kit provides all the necessary elements for efficient cloning of extra-long PCR products (up to 13 kb). The kit uses the linearized and … is medicare a single payer system