Phospho 4ebp1 antibody
Web4EBP1 is a 118 amino acid protein with a predicted molecular weight of 13 kD. Distribution Cytosol and nucleus/ubiquitously expressed Function Regulation of global translation Biology Area Cell Biology, Cell Proliferation and Viability, Protein Synthesis, Signal Transduction Molecular Family Phospho-Proteins Antigen References WebAnti-eIF4EBP1 (phospho T36) antibody See all eIF4EBP1 primary antibodies Description Rabbit polyclonal to eIF4EBP1 (phospho T36) Host species Rabbit Specificity ab47365 detects endogenous levels of 4E-BP1 only when phosphorylated at threonine 36. This antibody may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites.
Phospho 4ebp1 antibody
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Web(orb502277) 4EBP1 (phospho-Ser65/Thr70) antibody (Biotin) - Biorbyt - CiteAb A Biotin-conjugated rabbit polyclonal antibody, raised against Eukaryotic translation initiation factor 4E-binding protein 1 (Mouse), supplied by Biorbyt. WebThe Phospho-4EBP1 (Thr37/46) assay uses 2 labeled antibodies: one with a donor fluorophore, the other one with an acceptor. The first antibody is selected for its specific binding to the phosphorylated motif on the protein, the second for its ability to recognize the protein independent of its phosphorylation state.
Web人促胃液素受体(GsaR)ELISA试剂盒 Mouse proliferating cell nuclear antigen antibody,PCNA ELISA试剂盒 规格： 96T/48T. ... eIF4EBP1/4EBP1 eIF4E结合蛋白抗体 0.1ml 沙氏葡萄糖琼脂Sabouraud Dextrose Agar ... 磷酸化4E结合蛋白1抗体 0.1ml 沙氏葡萄糖肉汤Sabouraud Dextrose Broth phospho-eIF4EBP1(Thr37/46) 磷酸 ... WebNov 11, 2008 · To determine whether CDKs could be responsible for the rapamycin-stimulated rephosphorylation of 4EBP1, we treated cells with saturating concentrations of roscovitine (50 μM), a CDK inhibitor. ... Phospho-S6 and actin were blotted for normalization. (D) The experiments were completed in the same way as in C, except WT 4E-BP1, AA 4E …
WebPhospho-4E-BP1 (Ser65) Antibody detects endogenous levels of 4E-BP1 when phosphorylated at serine 65. This antibody has been shown to also recognize 4E-BP1 when phosphorylated at serine 101 (See application reference, Wang et al. 2003). Species …
WebMar 22, 2024 · Both phosphorylation sites of rpS6 (S235/236 and S240/244) were detected with phospho-specific antibodies. Total protein levels of rpS6, FLAG and β-actin were also detected with immunoblotting. b and c HEK293T and SW480 cells were transfected with FLAG empty vector or FLAG-DRAM1 plasmids for 24 h, and the cells were starved for …
WebAll lanes : Anti-eIF4EBP1 (phospho S65) antibody [EPR23889-3] at 1/1000 dilution Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate Lane 2 : HeLa treated with 100nM rapamycin for 24 hours, whole cell whole cell lysate Lysates/proteins at 20 µg per lane. Secondary All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at … floor and decor employee websiteWebRabbit anti 4EBP1 (pSer65) antibody recognizes eukaryotic translation initiation factor 4E-binding protein 1 (4EBP1) also known as phosphorylated heat-and acid-stable protein regulated by insulin 1 (PHAS-I), when phosphorylated at serine 65. 4EBP1 belongs to the eIF4E-binding protein family. 4EBP1, when bound to eIF4E, inhibits cap-dependent ... great neck musicWeb实验用到的供体是Thr46（铽标记的anti-phospho-4EBP1），受体是绿色荧光标记的4EBP1，是mTOR下游信号通路的蛋白，有活性的mTOR能磷酸化4EBP1，使之与供体结合。如果mTOR活性受到抑制，供体受体距离不能达到荧光能量转移的要求，则在515nm处与485nm处的信号比值增大。 floor and decor discontinued tileWebThe lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -2℃ . When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. 溶质携带物家族-1抗体， ... great neck music schoolWebOur 4EBP1 Antibodies can be used in a variety of model species: Human, Mouse, Porcine, Rat. Use the list below to choose the 4EBP1 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. great neck ncWebA Life Sciences and Diagnostics business focused on enabling scientists and healthcare professionals to advance research, discovery and detection to improve global health Analytical & Enterprise Solutions An Analytical and Enterprise solutions business, including leading OneSource laboratory services, focused on accelerating scientific outcomes great neck multi toolWebFeb 10, 2024 · Cell Signaling Technology's Phospho-4E-BP1 (Thr70) Antibody is a Rabbit Polyclonal antibody. This antibody has been shown to work in applications such as: Precipitation, Immunoprecipitation, and Western Blot. Phospho-4E-BP1 (Thr70) Antibody detects endogenous levels of 4E-BP1 only when phosphorylated at threonine 70. floor and decor engineered wood flooring